| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1251-1 | ||||
Resumo:Fungal infections, especially those caused by Candida spp. yeasts, have significantly increased in recent years. Candida is a commensal yeast present in various parts of the human body, becoming opportunistic and causing pathologies when the immune system is compromised. Candidiasis, an infectious disease caused by this fungus, has gained global prominence, with both superficial and invasive forms, including candidemia, resulting in high mortality rates. Due to the growing resistance to antifungal agents and the limited availability of antifungal drugs, there is a growing demand for novel antimicrobial agents. In this context, dimethyl sulfoxide (DMSO) is widely used as a solvent in assays for evaluating new antimicrobial compounds. Industrially, DMSO is obtained through chemical synthesis, with lignosulfonate being the main industrial source. The present study aimed to assess the effect of DMSO on the growth of four Candida spp. strains: C. albicans (ATCC 24433), C. glabrata (ATCC 2001), C. krusei (ATCC 20298) e C. parapsilosis (ATCC 22019). Growth curves were generated for each strain at different concentrations of DMSO. Results indicated that a 10% concentration of DMSO led to a significant decrease in growth and prolonged lag phase for all Candida spp. strains. At concentrations of 15% and 25% of DMSO, complete growth inhibition was observed for all evaluated species. In contrast, DMSO concentrations of 2.5% and 5% did not impact the growth pattern of the strains. These effects of DMSO on yeast growth can be attributed to its unique physicochemical properties, including its ability to dissolve compounds and enhance the action of antimicrobial agents. It is worth noting that DMSO concentrations below 10% have been reported to inhibit bacterial growth in other studies. In conclusion, DMSO demonstrated an impact on the growth of Candida spp. at concentrations starting from 10%, with complete growth inhibition at concentrations of 15% and 25%. Concentrations below 5% did not exert significant effects on yeast growth, suggesting that DMSO up to this concentration can be safely used as a solvent in yeast cultures and biological assays involving Candida. This study is relevant to ensure the accuracy of scientific research results involving Candida spp. and may contribute to industrial and biotechnological applications related to these microorganisms. Proper utilization of DMSO as a solvent is essential for obtaining reliable and promising outcomes in the quest for novel antifungal agents.
Palavras-chave: antimicrobial agents, dimethyl sulfoxide, fungal infections Agência de fomento:Fundação de Amparo à Pesquisa e Inovação do Espírito Santo – FAPES. | |||||